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. 2022 Jun 10;26:141–157. doi: 10.1016/j.omto.2022.06.005

Figure 5.

Figure 5

Caspase-mediated cell death in IFNα-sensitive cells

(A and C) Cell counts by Trypan blue dye exclusion method in MB49 (A) and UPPL1541 (C) cells treated with recombinant TRAIL (rTRAIL, 100 ng/mL) in the presence or absence of caspase 8 inhibitor (50 μM). rTRAIL reduced cell counts in a dose-dependent manner and was partially rescued by addition of caspase 8 inhibitor. (B and D) Percentage of apoptotic cells measured by annexin V/propidium iodide (PI) staining showed increased apoptosis in MB49 (B) and UPPL1541 (D) cells treated with rTRAIL and was rescued in cells treated with rTRAIL and caspase 8 inhibitor. (E) MB49 cells treated with increasing concentrations of tunicamycin, ER stress inducer, showed dose-dependent reduction in cell counts. (F) Caspase 12 inhibitor rescues cell death induced by rIFNα. (G and H) IL-6 protein expression measured by ELISA in cell-free supernatants of MB49 (G) and UPPL1541 (H) cells is shown. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001.