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. Author manuscript; available in PMC: 2022 Jul 4.
Published in final edited form as: Nat Protoc. 2022 Jan 12;17(2):421–444. doi: 10.1038/s41596-021-00648-5

Figure 2 |. Template matching.

Figure 2 |

(a) A typical tomographic slice (6 nm thick) depicting HIV-1 Gag T8I assemblies from the raw data. (b) The template used for particle picking, top and side views of HIV-1 Gag map (EMD-8403) low-pass filtered to 25 Å. (c) A tomographic slice of resulting convolution map overlaid with template matched model points of top cross-correlation peaks. (d) A projection view of model points through the tomogram volume. Scale bar is 50 nm.

- If found this confusing. What is the connection between the image in panel A and that shown in panel B?

- You mention “template search”. What template is used? If meaningful, could you add the template to the figure?