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. Author manuscript; available in PMC: 2022 Jul 4.
Published in final edited form as: Nat Protoc. 2022 Jan 12;17(2):421–444. doi: 10.1038/s41596-021-00648-5

Table 2 |.

emClarity processing run time (5 tilt-series).

emClarity processing steps Binning GPU card # GPU units Time

ctf estimate 1 Tesla V100 1 25 m
template search 8 Tesla V100 1 1 h
init cycle 0–2 6 Tesla V100 4 40 m
tomoCPR-1 cycle 3–5 5 Tesla V100 4 2.5 h
tomoCPR-2 cycle 6–8 4 Tesla V100 4 3 h
Classification 4 Tesla V100 4 40 m
cycle 9–10 4 Tesla V100 4 1.5 h
tomoCPR-3 cycle 11–13 3 Tesla V100 4 2 h
tomoCPR-4 cycle 14–16 2 Tesla V100 4 3 h
tomoCPR-5 cycle 17–18 1 Tesla V100 4 10 h
avg FinalAlignment 1 Tesla V100 4 1 h
cisTEM reconstruct/refine 1 Tesla V100 (cpu) 12 cpu cores 1.5 h

Note: Each tomogram is divided into multiple sub-regions (one VLP/sub-region), which are processed in parallel.