Skip to main content
. Author manuscript; available in PMC: 2022 Jul 4.
Published in final edited form as: Nat Protoc. 2022 Jan 12;17(2):421–444. doi: 10.1038/s41596-021-00648-5
System parameters:
nGPUs=4 %% number of visible GPUs
nCpuCores=12 %% maximum number of processes to run in parallel.
Microscope settings:
PIXEL_SIZE=1.179e-10 %% pixel size of raw tilt-series in meter
SuperResolution=0 %% whether raw tilt-series pixel size corresponds to super-resolution image pixel size
Cs=2.7e-3 %% in meter
VOLTAGE=300e3
AMPCONT=0.1 %% amplitude contrast
beadDiameter=7e-9 %% fiducial bead diameter in meter
Defocus range:
defEstimate=2.3e-6 %% in meter
defWindow=1.5e-6 %% in meter
Exposure-weighting parameters:
CUM_e_DOSE=123 %% total exposure dose
doseAtMinTilt=3 %% electron dose at minimum tilt
oneOverCosineDose=0 %% whether Saxon scheme is used
startingAngle=0 %% refined data collection starting angle
startingDirection=pos %% data collection direction
doseSymmetricIncrement=1 %% dose symmetric scheme group size