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. Author manuscript; available in PMC: 2022 Jul 4.
Published in final edited form as: Nat Protoc. 2022 Jan 12;17(2):421–444. doi: 10.1038/s41596-021-00648-5
Parameters:
Tmp_samplingRate=8 %% binning factor for tomogram for template Search
particleRadius=[66,66,56] %% in Angstrom. cross-correlation peak radius to remove from consideration after a particle in the current peak is selected.
Ali_mRadius=[116,116,72] %% Radius of alignment mask in Angstrom
Tmp_angleSearch= [180,9,35,7] %% in degrees
Tmp_threshold=1000 %% estimate number of particles
symmetry=C6 %% particle symmetry