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. 2022 Jun 21;66(3):3391. doi: 10.4081/ejh.2022.3391

Figure 2.

Figure 2.

A) Immunohistochemistry for β-gal; original magnification 40×, scale bar: 25 μm. B) Semi-quantitative analyses showed a significant increase in β-gal in WSD-fed mice compared to the SD group. C) Immunostaining for β-gal (IF, green) and Tunel (IHC, brown) in WSD-fed mice compared to the SD group; the staining confirmed the activation of the apoptosis-resistant senescent pathway; nuclei are counterstained with DAPI (IF, blue); original magnification 60×, scale bar 15 μm. These images are representative of n=8 SD, and n=8 WSD mice. Data were analyzed using the Mann-Whitney test; SD, standard diet; WSD, Western-style diet.