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. 2022 Jun 21;66(3):3391. doi: 10.4081/ejh.2022.3391

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Figure 3. — A,C) Immunohistochemistry for p53, and p-H2AX; upper, original magnification 20×, scale bar: 50 μm; inset, scale bar: 50 μm. B,D) The expression of p53 and p-H2AX was significantly increased in WSD mice compared to the SD group, as shown by semiquantitative analyses. E G) Immunohistochemistry for IL-6 and MMP1; original magnification 20×, scale bar: 50 μm. F,H) Semi-quantitative analyses showed a significant increase in both IL-6 and MMP1 in WSD-fed mice compared to the SD group. Values represent means ± standard deviation. Data were analyzed using the Mann-Whitney test; SD, standard diet; WSD, Western-style diet.