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A) Immunofluorescence for β-gal (green) and Gpx4 (red) showed an increase in β-gal/Gpx4-positive cells in WSD-fed mice compared to the SD group; Nuclei are counterstained with DAPI (blue); original magnification 20×, scale bar: 50 μm. B) Quantification of A. These images are representative of n=8 SD, and n=8 WSD mice. Data were analyzed using the Mann-Whitney test; SD, standard diet; WSD, Western-style diet.
