16S rDNA slot blot analyses of PCR products of agrobacterium collection with the biovar 1 cluster DNA probes F639rrsAT41 and F640rrsAT42 (a) and the biovar 2 DNA probe F641rrsAR5 (b). See Table 1 for a description of the strains. Biovar 1 DNAs were obtained from strains CFBP2413T (Spot A1), Ach5 (B1), CFBP1903 (C1), GMI9023 (D1), CFBP1901 (E1), CFBP296 (F1), CFBP354 (G1), CFBP1904 (H1), CFBP2241 (A2), CFBP2243 (B2), CFPB2407 (C2), CFBP2410 (D2), CFBP2411 (E2), CFBP2414T (F2), CFBP2454 (G2), CFBP2456 (H2), CFBP2457 (A3), CFBP2458 (B3), CFBP2177 (C3), CFBP2516 (D3), CFBP2518 (E3), S56 (F3), S377 (G3), S4 (H3), ATCC 4720 (A4), CIP28-75 (B4), CIP43-76 (C4), CIP127-76 (D4), CIP111-78 (E4), NCPPB925 (F4), F/1Zutra (G4), RV3 (H4), 3/1Zutra (A5), NCPPB1641 (B5), T37 (C5), ICPB TT9 (D5), 6 Mushin (E5), O362 (F5), O363 (G5), A96.11 (H5), A134.2 (A6), A134.3 (B6), A134.6 (C6), B100.11 (D6), M9 (E6), M10 (F6), M15 (G6), M214 (H6), M292 (A7), X88.283 (B7), X88.293 (C7), X88.299 (D7), X88.303 (E7), 85.2 (F7), 85.6 (G7), 85.49 (H7), 85.52 (A8), 85.66 (B8), and 85.104 (C8). A. fici DNA was obtained from strain AF3.44; (E8). A. rubi DNA was obtained from strain CFBP999T (G8). Biovar 2 DNAs were obtained from strains CFBP450 (A9), CFBP1804 (B9), CFBP1905 (C9), CFBP1936 (D9), CFBP1937 (E9), CFBP1961 (F9), CFBP1962 (G9), CFBP2178 (H9), CFBP2408T (A10), CFBP2417 (B10), CFBP2418 (C10), CFBP2419 (D10), CFBP2519 (E10), CFBP2520 (F10), C104.12 (G10), C104.22 (H10), M3 (A11), M32 (B11), M84 (C11), M111 (D11), M120 (E11), 85.30 (F11), 85.110 (G11), and 85.120 (H11). Biovar 3 DNAs were obtained from strains CFBP2512 (A12), CFBP2618 (B12), CFBP2620 (C12), CFBP2621 (D12), and CFBP2622 (E12).