TABLE 1.
Agrobacterium strains used in this study
| Straina | Opine type produced in tumorb | Host plant or other properties | Primary designation and/or origin | MIC (mg · liter−1) of:
|
16S rDNA probee | Hybridization with plasmid Ti DNA probesf
|
||||
|---|---|---|---|---|---|---|---|---|---|---|
| tmr | nos |
vir
|
||||||||
| Na2SeO3c | K2TeO3d | Plasmid Ti nopaline type | Plasmid Ti octopine type | |||||||
| Biovar 1 | ||||||||||
| Pathogenic strains | ||||||||||
| CFBP2413T | O/M | Apple tree | B6, United States | 8,000 | 320 | 1 | + | − | + | + |
| Ach5 | O/M | Prunus sp. | United States (Y. Dessaux, France)h | 8,000 | 320 | 1 | + | − | + | + |
| CFPB2407 | C/O | Vitis vinifera cv. Danom | France | 8,000 | 320 | 1 | + | − | + | − |
| CFBP1903 | N | Prunus cerasus | C58, New York | 8,000 | 320 | 1 | + | + | + | + |
| CFBP1901 | N | C58 with pTiT37 | (Y. Dessaux, France)h | 8,000 | 320 | 1 | + | + | + | + |
| CFBP354 | N | ND | B10, France | 8,000 | 160 | 1 | + | + | + | + |
| CFBP1904 | N | Vitis vinifera | AG20, Greece | 8,000 | 320 | 1 | + | + | + | + |
| CFBP2410 | N | Populus sp. | M22, ND | 8,000 | 160 | 1 | + | + | + | + |
| CFBP2411 | N | Salix purpurea | CG4, New Zealand | 8,000 | 160 | 1 | + | + | + | + |
| CFBP2177 (6) | N | Populus section Leuce | France | 8,000 | 160 | 1 | + | + | + | + |
| CFBP2516 (5) | N | Populus section Leuce | France | 8,000 | 320 | 1 | + | + | + | + |
| A96.11 | N | Populus section Leuce | France | ND | ND | 1 | + | + | + | + |
| A134.2 (5) | N | Populus section Leuce | France | ND | 160 | 1 | + | + | + | + |
| M10 (4) | N | Populus section Leuce | France | ND | 320 | 1 | + | + | + | + |
| S56 | M | NDg | France (G1)i | ND | 160 | 1 | − | − | − | − |
| S377 | ND | ND | France (G1)i | ND | 160 | 1 | − | − | − | − |
| S4 | N | Black raspberry | France (G1)i | ND | 160 | 1 | + | + | + | + |
| ATCC 4720 | ND | ND | United States (G1)i | ND | 160 | 1 | + | − | − | − |
| NCPPB925 | ND | Dahlia sp. | South Africa (G6)i | ND | 160 | 1 | − | − | − | − |
| F/1Zutra | ND | Dahlia sp. | Israel (G6)i | ND | 160 | 1 | + | − | − | − |
| 3/1Zutra | ND | Apple tree | Israel (G7)i | ND | 160 | 1 | − | − | − | − |
| NCPPB1641 | ND | Flacourtia ramontchi | United Kingdom (G7)i | ND | 160 | 1 | − | − | − | − |
| T37 | N | Walnut | United States (G8)i | ND | 160 | 1 | + | + | + | + |
| ICPB TT9 | ND | Hop | United States (G8)i | ND | 160 | 1 | + | + | + | + |
| 6 Mushin | ND | Hop | Australia (G8)i | ND | 160 | 1 | + | + | + | + |
| O362 | ND | Soil | South Australia (G9)i | ND | 160 | 1 | + | + | + | + |
| 2T3Pb (3) | ND | Walnut | Spain (M. Lopez, Spain)h | ND | 160 | 1 | + | + | + | + |
| 6MS3 | ND | Walnut | Spain (M. Lopez, Spain)h | ND | 320 | 1 | + | + | + | + |
| 436.3SA | M | Prunus hybrid cv. GF677 | Spain (M. Lopez, Spain)h | ND | 320 | 1 | + | − | + | + |
| CFBP296 | N | Lycopersicon esculentum | 111, France | 8,000 | 160 | 1 | − | − | − | − |
| M15 | N | Populus section Leuce | France | 8,000 | 160 | 1 | − | − | − | − |
| Nonpathogenic strains | ||||||||||
| CFBP2414T | NAj | Unknown | 3-24-2, The Netherlands | 8,000 | 160 | 1 | − | − | − | − |
| C58C1 | NA | C58 cured of pTiC58 | 8,000 | 160 | 1 | − | − | − | − | |
| GMI9023 | NA | C58 cured of pTiC58 and pAtC58 | (Y. Dessaux, France)h | 8,000 | 160 | 1 | − | − | − | − |
| CFBP2518 | NA | Populus section Leuce | France | 8,000 | 160 | 1 | − | − | − | − |
| O363 | NA | Soil | Australia (G9)i | ND | 160 | 1 | − | − | − | − |
| CFBP2456 | NA | ND | CDC B6016, United States | ND | ND | 1 | − | − | − | − |
| CFBP2457 | NA | ND | CDC B3771, United States | ND | ND | 1 | − | − | − | − |
| RV3 | NA | ND | ND (G7)i | ND | 80 | 1 | − | − | − | − |
| CIP28-75 (3) | NA | Human | France (G2)i | ND | ND | 1 | − | − | − | − |
| CIP111-78 | NA | Human | France (G3)i | ND | ND | 1 | − | − | − | − |
| CFBP2454 | NA | Human | France | ND | ND | 1 | − | − | − | − |
| CFBP2458 | NA | ND | M2/1, ND | 8,000 | 160 | 1 | − | − | − | − |
| CFBP2241 | NA | Human | CDC 7258, ND | ND | ND | 1 | − | − | − | − |
| CFBP2243 | NA | Human | CDC A6597, United States | ND | ND | 1 | − | − | − | − |
| Biovar 2 | ||||||||||
| Pathogenic strains | ||||||||||
| CFBP2408T | A/M | ND | ND | ND | ND | 2 | − | − | + | + |
| CFBP450 | ND | Malus pumila cv. M IX | France | 2,000 | 1,280 | 2 | − | − | − | − |
| CFBP1804 | N | Prunus persicae cv. GF305 | France | 4,000 | 1,280 | 2 | + | + | + | + |
| CFBP1905 | N | Vitis vinifera | AG28, Greece | 4,000 | 1,280 | 2 | + | + | + | + |
| CFBP1936 | N | Rosa sp. | Tahiti | 2,000 | 1,280 | 2 | + | + | + | + |
| CFBP1961 | N | Populus bolleana | France | 4,000 | 1,280 | 2 | + | + | + | + |
| CFBP1962 | N | Prunus cerasus cv. Mahaleb | Spain | 4,000 | 1,280 | 2 | + | + | + | + |
| CFBP2178 | N | Prunus aviumcv. F12-1 | France | 12,000 | 1,280 | 2 | + | + | + | + |
| CFBP2417 (3) | N | Cherry hybrid cv. Colt | France | 6,000 | 1,280 | 2 | + | + | + | + |
| CFBP2519 (3) | N | Populus section Leuce | France | 4,000 | 1,280 | 2 | + | + | + | + |
| C104.12 | N | Populus section Leuce | France | 6,000 | 1,280 | 2 | + | + | + | + |
| C104.22 (8) | N | Populus section Leuce | France | ND | 1,280 | 2 | + | + | + | + |
| M120 | N | Populus section Leuce | France | ND | 640 | 2 | + | + | + | + |
| Nonpathogenic strains | ||||||||||
| CFBP1937 | NA | Soil | K84, Australia | ND | ND | 2 | − | − | − | − |
| CFBP2520 | NA | Populus section Leuce | France | 4,000 | 640 | 2 | − | − | − | − |
| Biovar 3 | ||||||||||
| Pathogenic strains | ||||||||||
| CFBP2512 | N | Vitis vinifera | 565-5, Spain | 10,000 | 1,280 | − | + | + | + | (+) |
| CFBP2620 | N | Vitis vinifera | K374, Australia | 14,000 | 1,280 | − | + | + | + | (+) |
| CFBP2618 | C/O | Vitis vinifera cv. Cabernet | 85-255, France | 8,000 | 1,280 | − | + | − | + | − |
| CFBP2622 | C/O | Vitis vinifera cv. Sultana | Ag63, Greece | 14,000 | 1,280 | − | + | − | + | (+) |
| CFBP2621 | C/O | Vitis vinifera cv. Sultana | Ag57, Greece | 14,000 | 1,280 | − | − | − | − | − |
| A. rubi | ||||||||||
| Pathogenic strain | ||||||||||
| CFBP999T | N | Rubus sp. | TR2, United States | 8,000 | 160 | 1 | + | + | + | + |
| A. fici | ||||||||||
| AF3.44 | Ficus benjamina | United States (H. Bouzar, United States) | 8,000 | 320 | 1 | ND | ND | ND | ND | |
CFBP, Collection Française de Bactéries Phytopathogènes, Institut National de la Recherche Agronomique, Angers, France; NCPPB, National Collection of Plant Pathogenic Bacteria, Harpenden, United Kingdom; ICPB, International Colleciton of Phytopathogenic Bacteria, Davis, Calif.; CIP, Collection de l'Institut Pasteur, Paris, France; ATCC, American Type Culture Collection, Manassas, Va. The numbers in parentheses indicate the numbers of strains with the same origin and the same relevant characteristics that gave the same MIC and hybridization results. The groups of strains were as follows: CFBP2177, B100.11, M9, 85.2, 85.6, and 85.104; CFBP2516, A134.6, M292, X88.283, and 85.66; A134.2, A134.3, M214, X88.299, and 85.49; M10, X88.293, X88.303, and 85.52; 2T3Pb, 1C3Pb, and 2T3Sa; CIP28-75, CIP43-76, and CIP127-76; CFBP2417, CFBP2418, and CFBP2419; CFBP2519, 85.100, and 85.186; and C104.22, M3, M32, M84, M111, 85.30, 85.120, and 85.123.
O/M, octopine/mannopine; N, nopaline; M, mannopine; C/O, cucumopine/octopine; A/M, agropine/mannopine.
All Se MICs were determined on MG medium.
Te MICs were determined by using 1A medium for biovar 1, 2E medium for biovar 2, and MG medium for biovar 3, A. rubi, and A. fici.
1, hybridization with the biovar 1 cluster chromosomal probes; 2, hybridization with the biovar 2 chromosomal probe for 16S DNA analysis.
+, hybridization; −, no hybridization; (+), weak hybridization.
ND, not determined.
The information in parentheses indicates the person who provided the strain.
G1 to G3 and G6 to G9 correspond to genomic species defined by Popoff et al. (31).
NA, not applicable.