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. Author manuscript; available in PMC: 2022 Jul 4.
Published in final edited form as: Nat Chem Biol. 2021 Dec 22;18(2):152–160. doi: 10.1038/s41589-021-00917-0

Figure 4. Modeling predicts BcLOV-SOScat-induced ppErk dynamics and reveals dynamic filtering properties of Ras/Erk signaling.

Figure 4.

A) A model of ppErk activation was developed by integrating the BcLOV4 membrane translocation model with a transfer function model that describes the input/output response of SOScat membrane localization (input) to ppErk activation (output). B) Filtering properties of a 1° vs 2° low pass filter (LPF). 1° LPFs attenuate high frequency inputs less than 2° low pass filters. C) A 1° LPF with 2 mHz cutoff frequency best describes ppErk dynamics when stimulated with fast 2’ON/2’OFF BcLOV-SOScat oscillations. Data points are the mean +/− SD of three biologically independent replicates, with each replicate representing the mean of ~1000-2000 cells. D) Heat map depicts the predicted Erk activation dynamics resulting from BcLOV4 membrane translocation dynamics over the indicated light and temperature conditions. Plots show model predictions of Erk activation at the indicated experimental conditions, and data points show experimental results. Datapoints represent means +/− SEM of ~1000-4000 cells. Unnormalized plots are presented in Supplementary Figure 14. See Methods for model details.