FIG. 4.

Location of Vvn in V. vulnificus. Proteins in the various cell fractions prepared from a 6-h culture of V. vulnificus YJ016 were separated by electrophoresis on a 12.5% polyacrylamide gel containing herring sperm DNA (20 μg/ml). The gel was then stained with Coomassie blue (left) or renatured, stained with ethidium bromide, and visualized with UV light (right). Lanes: M, molecular weight standards; 1, purified Vvn (a gift from C. M. Hsu), 4 μg; 2, periplasmic fraction, 10 μg; 3, cytoplasmic extract, 10 μg; 4, culture supernatant, 10 μg.