FIG. 5.

Detection of the deletion in vvn in the chromosome of V. vulnificus. (A) Restriction map of vvn with flanking regions. The deletion (blank bar), the two pairs of primers used to amplify the two sequences that, when ligated, generated a 335-bp deletion in vvn, and the probe used in Southern hybridization are indicated. (B) Southern hybridization. Ten-microgram aliquots of total DNA of various V. vulnificus strains were double digested with MluI and HpaI and then subjected to electrophoresis on a 1.2% agarose gel. Lanes: M, DNA molecular weight standards; 1, YJ016; 2, YJ016; with pSK002 integrated in the chromosome; 3, SK005 (YJ016 Δvvn).