Skip to main content
. 2022 Jun 20;13:905966. doi: 10.3389/fphar.2022.905966

FIGURE 2.

FIGURE 2

Curcumenol inhibited the phosphorylation of the NFκB pathway and up-regulation of the MMP family in vitro. (A–D) RT-qPCR analysis of the relative mRNA expression levels of Col2a1, MMP3, MMP9, and MMP13 in NP cells stimulated with TNFα (10 ng/ml) and different range of Curcumenol (0, 6.25, 12.5, 25, and 50 μM) for 24 h. (E) Western blot analysis of p-P65, P65, p-IκBα, and IκBα expression in NP cells pretreated with 50 μM Curcumenol and then stimulated with TNFα (10 ng/ml) for 10 min. (F) Western blot analysis of MMP3, MMP9, and MMP13 expression in NP cell stimulated with TNFα (10 ng/ml) or/and 50 μM Curcumenol for 24 h. (G) Semi-quantification of grey scale value in MMP3, MMP9, and MMP13 was conducted using β-actin as the reference in panel F. (H) Semi-quantification of grey scale value in p-P65/P65 and p-IκBα/IκBαwas conducted in panel E. All data are presented as mean ± SD from three replicates. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. p-, phosphorylated; Col2a1, collagen type II α 1 chain.