FIGURE 3.

Curcumenol mitigated the inflammation induced by TNFα in Rat’s IVDs ex vivo model. (A) H&E and Safranin O-Fast Green staining of paraffin sections of the rat’s intervertebral discs ex vivo model at a coronal position. (B) Immunofluorescence analysis for phosphorylation and translocation of P65 in NP cells pretreated with 50 μM Curcumenol and stimulated with TNFα (10 ng/ml) for 20 min. (C) Immunohistochemistry analysis of TNFα and IL-1β expression in ex vivo model at a coronal position. (D) Quantification of histological score in the sections described in panel A. (E) Quantification of positive cells in the sections described in panel (C) *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001.