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Flow cytometric analysis of HSPC cells (LSK: Lineage-, Sca1+, cKit+) from whole bone marrow cells in BMME-on-Chip. (A) Gating strategy for analysis of LSK cells using FlowJo v10. (B) Percent of LSK in live cells population in the chip on D21 and control C57Bl/6J BM at 6–8 weeks of age (Mean, SD). Statistical analysis was performed using an unpaired t-test. **: p ≤ 0.01. BM: Bone marrow.
