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Diagram of ex vivo culture of sorted LSK cells in BMME-on-chip and competitive transplant. (A) CD45.2+ LSK cells were sorted using flow cytometry and cultured with BMSC in hydrogel for 14 days in BMME-on-chip. (B) Functional analysis using competitive transplant assay of bone marrow and chip grown LSK CD45.2+ donor into recipient B6 CD45.1 (C) with CD45.1+ competitor cells. Illustrated using Biorender.com.
