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. 2022 Jun 20;10:855777. doi: 10.3389/fbioe.2022.855777

FIGURE 6.

FIGURE 6

Engraftment and repopulation of HSPC, myeloid progenitor, and lymphoid precursor populations in bone marrow by BMME-on-chip grown LSK cells. (A) Bone marrow of recipient mice was isolated and analyzed using flow cytometry for lineage markers, stem cell markers Sca1+ and cKit+. (B–E) Representative contour plots of CD45.1+ and CD45.2+ in LSK (Lin-cKit + Sca1+), myeloid (lin-cKit+), and lymphoid (lin-Sca1+) cells in bone marrow of (B) CD45.2+ C5BL/6J, (C) B6 CD45.1+, (D) chip transplant recipient, and (E) mouse marrow control recipient. (F) Quantification of total bone marrow cells after isolation. (G) Viability isolated bone marrow cells using flow cytometry (negative DAPI staining). Statistical analysis for F and G was performed using an unpaired t-test. ns = no significance, error bar = SD. (H–J) Chimerism of LSK (H), myeloid (I), and lymphoid (J) cells indicating repopulation of CD45.2+ donor cells and competitor CD45.1+ cells in recipient mice. Data show the percent of total cells analyzed using flow cytometry of bone marrow from each recipient mouse. In particular, control recipients M1, M4, and M5, and chip recipients C1, C4, and C5 show chimerism in LSK populations.