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. 2022 Jun 20;10:855777. doi: 10.3389/fbioe.2022.855777

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Copyright © 2022 Sharipol, Lesch, Soto and Frisch.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Repopulation of myeloid-biased MPP2+ and MPP3+, lymphoid-biased MPP4+ multipotent progenitor and HSC populations by BMME-on-chip donor cells. Subpopulations of specific progenitor cells and short-term and long-term repopulating HSC (ST-HSC and LT-HSC) within the LSK populations were analyzed for mice recipients with CD45.2+ and CD45.1+ chimerism (M1, M4, M5, C1, C4, and C5). (A) Flow cytometry gating of LSK subpopulations. (B–K) Representative contour plots and quantification of donor and competitor cells in LSK subpopulations. Statistical analysis was performed using multiple Whitney–Mann tests. N = 3 per group. Error bars = standard deviation, ns = non-significant.