FIGURE 8.

Repopulation of peripheral blood population in secondary transplant recipients shows maintenance of long-term HSPC function after BMME-on-chip culture. (A) Diagram of secondary transplant of lethally irradiated mice with bone marrow cells from primary recipients at 28 weeks post-transplant. Peripheral blood was analyzed using flow cytometry at 4w and 8w post-secondary transplant. (B,C) Flow cytometry data showing chimerism of CD45.2+ donor cells in total live cells in peripheral blood at 4w and 8w. Consistent with bone marrow engraftment data, C2 and C3 do not have CD45.2+ engraftment. (D–F) Flow analysis of total cells, monocytes, and lymphocytes from chip recipients with CD45.2+ engraftment (C1, C4, and C5) compared to mouse recipients (C1, C4, and C5). Flow analyses were performed using FlowJo V10. Statistical analysis for (D–F) was performed using an unpaired t-test. N = 3. ns = non-significant.