Figure 2.
Formation of isolated reaction sites and the microarray synthesis approach. (A) Droplet formation by filling and draining a PGA-P solution in a microarray reactor for the subsequent PGA-gated deprotection reaction. (B) A microscopic image of CH3OH droplets formed on a microarray glass plate patterned by a non-wetting film. (C) Examples of digital light projection patterns generated on a desktop computer for selective PGA deprotection. Four masks are required for the A, C, G or T residue per step growth in oligonucleotide array synthesis. At irradiated circles (white), light is controllable (in terms of irradiation time and strength). Alignment marks are on the left and right side of the digital masks.