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. 2022 Jun 20;13:889991. doi: 10.3389/fimmu.2022.889991

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Copyright © 2022 He, Chen, Hao, Yang and Shang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sequence alignment and phylogenetic analysis of chicken IL-9 with the counterparts of other vertebrates. The GenBank accession numbers of IL-9 proteins are chosen as follow: chicken (Gallus; ACY79396.1), budgerigar (Melopsittacus undulates; XP_012984011.2), human (Homo sapiens; AAC17735.1), green monkey (Chlorocebus sabaeus; ACI28916.1), mouse (Mus musculus; NP_032399.1), rat (Rattus norvegicus; NP_001099217.1), cat (Felis catus; XP_003980942.1), dog (Canis lupus familiaris; XP_003431641.1), horse (Equus caballus; XP_001504404.1), pig (Sus scrofa; NP_001159515.1). The GenBank accession numbers of outgroup cytokine IL-4 include chicken (Gallus; CAF18427.1), duck (Anas platyrhynchos; ATP76495.1), human (Homo sapiens; NP_000580.1), mouse (Mus musculus; AAA39298.1), and pig (Sus scrofa; ADZ24282.1). (A) The sequence alignment of IL-9 was performed with BioEdit software with CLUSTAL W algorithm. (B) The phylogenetic analysis of chIL-9 and chIL-4 with other animals was performed with MEGA 6 software. The maximum likelihood tree was constructed and Bootstrap support values are shown for each node. The scale bar indicates the number of amino acid substitutions per site.