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. 2022 Jun 20;13:889991. doi: 10.3389/fimmu.2022.889991

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Copyright © 2022 He, Chen, Hao, Yang and Shang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Recombinant chIL-9 was expressed in DF-1 cells. Recombinant plasmid pEGFP-chIL-9 was transfected into DF-1 cells and the expression of recombinant chIL-9 were detected by Immunocytochemistry (ICC) and Western Blot. (A) ICC showed that recombinant chIL-9 was expressed in pEGFP-chIL-9-transfected (right panel) but not pEGFP-transfected (left panel) DF-1 cells and recognized specifically by mouse anti-chIL-9 polyclonal antibodies (pAb) (lower panel). (B) Recombinant chIL-9 was detected by Western Blot in the whole cell lysate (left panel) and supernatant (right panel) of DF-1 cells that were transfected with pEGFP-chIL-9 plasmid (lane 2 and 4) but not pEGFP-C vector (lane 1 and 3).