Figure 2.

Proteomics and glycosyl linkage analysis of S. indica EPS matrix and CW. A, Venn diagram of proteins identified in the EPS matrix, CW, and/or culture filtrate from three cultivation media (CM, YPD, and TSB). B, Proteins with WSC domain/s show higher abundances in the EPS matrix of S. indica (see also Supplemental Figure S2 and Supplemental Data Sets S1, S2, and S3). The relative abundance of each protein was calculated using LFQ intensity values and is depicted in percentage. C, Glycosidic linkage analysis of S. indica EPS and CW preparations. 3-glucose and 3,6-glucose are abundant in the EPS matrix, whereas 4-glucose is abundant in the CW of S. indica. The experiment was performed with four independent biological replicates of Si EPS and Si CW and the TIC of one of the replicates is represented. TIC, total ion chromatogram; LysM, lysine motif; p, pyranose; Si, Serendipita indica.