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. 2022 Jun 7;11:e69031. doi: 10.7554/eLife.69031

Table 2. Barbed end, branch and filament number for fibroblast cells in Vinzenz et al., 2012 and keratocyte cells from Mueller et al., 2017 for lamellipodia region of 1µm.

Since the leading edge is not well defined in the EM tomograms, we consider the leading edge to begin at the maximum barbed end value but we also include the number of barbed ends that would be considered outside the cell (with this definition) in the region of 0–0.212 µm.

Quantity Fibroblast Fibroblast Keratocyte Keratocyte
Region (µm) Value (µm-1) Region (µm) Value (µm-1)
Barbed ends (first bin) 0–0.25 145 0–0.212 309
Barbed ends (second bin) 0.25–0.5 42.5 0.212–0.424 238
Branches (first bin) 0–0.25 37.5 N/A N/A
Branches (second bin) 0.25–0.5 37.5 N/A N/A
Pointed ends (first bin) N/A N/A 0–0.212 91
Pointed ends (second bin) N/A N/A 0.212–0.424 231
Filaments (first bin) 0–0.25 150 0–0.106 200
Filaments (second bin) 0.25–0.5 130 0.106–0.212 256