Table 1. Sequences of oligodeoxyribonucleotide primers used in PCR experiments.
Primer | Sequence | Primer position on template mRNA | |
---|---|---|---|
(5′) | (3′) | ||
Tag-1 | GCCAGTTTAAACGAT | ||
Tag-2 | CUAAUACGACUCACUAUAGGG | ||
Ω | ATATGAATTCGTATTTTTACAACAATTACC | TMV (1–20) | |
U1-Sph | ATATCCATGGTCTCGAACGTCCAGGTTGGGC | TMV (449–461) | |
K5 | ATAAGAATTCGTTTTAGTTTTATTGCAACAA | crTMV (1–21) | |
2PM | CTCAAGCGATCGAAAGCCA | crTMV (323–339) | |
α-R-5′ | GGTCCAGTCCGACTGAG | α-Globin (9–25) | |
α-R-3′ | CTCACTCAGACTTTATTC | α-Globin (530–547) | |
β-R-5′ | CTTTTGACACAACTGTG | β-Globin (8–24) | |
β-R-3′ | CCAGAAGTCAGATGCTC | β-Globin (546–563) | |
G-5′ | TTAGCACCCCTGGCCAAGG | GAPDH (529–547) | |
G-3′ | CTTACTCCTTGGAGGCCATG | GAPDH (1050–1069) |