Table 2.

Primers for detection of colistin-resistant genes.

Target gene	Primer	Sequence (5′-3′)	Amplification product (bp)	Annealing temperature (°C)	Reference
mcr-1	mcr-1F	ATCAGCCAAACCTATCCCATCG	1257	55	[18]
	mcr-1R	GCAGACGCACAGCAATGCCTAT
mcr1_320 bp	mcr1_320 bp_F	AGTCCGTTTGTTCTTGTGGC	320	58	[19]
	mcr1_320 bp_R	AGATCCTTGGTCTCGGCTTG
mcr-2	mcr-2F	GCGATGGCGGTCTATCCTGTAT	378	55	[18]
	mcr-2R	TGCGATGACATGGGGTGTCAGC
mcr-2_700 bp	mcr2_700 bp_F	CAAGTGTGTTGGTCGCAGTT	715	58	[19]
	mcr2_700 bp_R	TCTAGCCCGACAAGCATACC
mcr-3	mcr-3F	TATGGGTTACTATTGCTGG	814	55	[18]
	mcr-3R	CTACCCTGATGCTCATCG
mcr3_900 bp	mcr3_900 bp_F	AAATAAAAATTGTTACGCTTATG	929	58	[19]
	mcr3_900 bp_R	AATGGAGATCCCCGTTTTT
mcr-4	mcr-4F	GTCATAGTGGTATAAAAGTACAG	669	55	[18]
	mcr-4R	CCACCGTCTATCAGAGCCAAC
mcr_4_1100 bp	mcr_4_1100 bp_F	TCACTTTCATCACTGCGTTG	1116	58	[19]
	mcr_4_1100 bp_R	TTGGTCCATGACTACCAATG
mcr-5	mcr5_F	ATGCGGTTGTCTGCATTTATC	1644	58	[20]
	mcr5_R	TCATTGTGGTTGTCCTTTTCTG
mmcr-5	mcr-5F	GCGGTTGTCTGCATTTATCAC	1042	50	[18]
	mcr-5R	CTTTGAAAACCTGTCTTTGGCA
mcr-6	mcr-6F	GTCCGGTCAATCCCTATCTGT	556	55	[18]
	mcr-6R	ATCACGGGATTGACATAGCTAC
mcr-7	mcr-7F	TGCTCAAGCCCTTCTTTTCGT	892	55	[18]
	mmcr-7R	TTCATCTGCGCCACCTCGT
mcr-8	mcr-8F	AACCGCCAGAGCACAGAATT	667	60	[18]
	mcr-8R	TTCCCCCAGCGATTCTCCAT