Figure 2.

NMR ensemble complex (PDB code: 2LPB) of the mediator coactivator domain 1 of Gal11/med15 (Chain A, green) bound to the central activation domain of Gcn4 (Chain B, orange). The following panels are shown on the top half: Structural ensemble composed of 13 different conformations (left). Summary of inter-chain contacts with a frequency greater than 50% in all models found by RING (center). Resulting inter-contact network with edges and nodes colored by chain and interaction type (right). The following panels are shown in the bottom half: Key Gcn4 residues interactions that dynamically bind Gal11 in one conformation (left). Range of contact frequency in which these inter-chain interactions are found (center). Resulting inter-contact network highlighting edges and nodes involved in the protein-protein interface (right).