Fig. 1.

Short-term administration of olanzapine and metformin modulated hypothalamic neuropeptides without altering body weight and food intake in female mice. (A) Experimental scheme in female mice that were orally injected with vehicle, OLZ, and OLZ + MET (n = 4-5). (B) Body weights of mice that were treated with OLZ and OLZ + MET for 21 days (n = 4-5). (C) Average daily food intake before the onset of obesity (n = 4-5). (D) Effect of OLZ and OLZ + MET treatment on hypothalamic Pomc, Npy, and Agrp mRNA expression. Data are presented as mean ± SEM values. Arrow indicates the time point of analysis. Statistical analyses were performed using one-sided two-way ANOVA (B), and one-sided one-way ANOVA (C, D) followed by a post hoc LSD test. *P (CON vs OLZ) and ^†P (OLZ vs. OLZ + MET) < 0.05, and ***P < 0.001 between the indicated groups.