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. 2022 May 25;36(7):1834–1842. doi: 10.1038/s41375-022-01607-z

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — CFs were calculated and provided to laboratories on an annual basis. The stability of each CF was determined as either optimal (bright green), satisfactory (green) or unvalidated (amber) by comparison with the previous year’s value using the following criteria; Optimal (+/− 1.2 fold): Old CF/New CF = 0.83–1.2, Satisfactory (+/− 1.6 fold): Old CF/New CF = 0.63–1.58 or Unvalidated: Old CF/New CF < 0.63 or >1.58. The bars charts show the number of laboratories for each category, per year for GUSB reference gene data sets. Several laboratories submitted data for more than one reference gene or assay and therefore the number of data sets analysed may be greater than the number of participating laboratories.