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. 2022 May 21;36(7):1916–1925. doi: 10.1038/s41375-022-01598-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Scheme of the generation of polyclonal v-ABL⁺ cell lines (upper panel) and representative FACS plots of B-cell marker staining (lower panels). Live cell population of v-ABL⁺ wild type and p27-Y88F cell lines were gated for CD19⁺ cells followed by B220 and CD43 as indicated by arrows. B Immunoblot analysis of wild type and p27-Y88F v-ABL⁺ cell lines generated from leukemic bone marrow cells shows protein levels of p27, SKP2, v-ABL, pY-CRKL, pY-STAT5A/B, p-ERK1/2, MYC, p53, p21 and GAPDH and HSP90 as loading controls. Densitometric analyses of p27 normalized to GAPDH, SKP2 and v-ABL levels normalized to HSP90 are shown below. Protein levels in wild type cells were set to 1. C Percentages of v-ABL⁺ wild type and p27-Y88F cell lines in G1, S and G2/M phase (left panel) and sub-G1 phase (right panel) as determined by flow cytometry. Lower panel displays p27 mRNA levels determined by qPCR in wild type and p27-Y88F v-ABL⁺ cell lines. n = 4 per genotype. Cell lines correspond to those used in B. One representative experiment out of three is shown. Data represent mean ± SD. (*p < 0.05, **p < 0.01, ***p < 0.001).