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. 2022 May 21;36(7):1916–1925. doi: 10.1038/s41375-022-01598-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A1 Left graph represents cell cycle phase distribution of v-ABL⁺ wild type and p27-Y88F cells as determined by flow cytometry after propidium iodide and anti-BrdU staining. Right graph indicates percentages of live cells identified by flow cytometry as cells staining negative for Annexin V and DAPI. A2 Colony formation assay of wild type and p27-Y88F v-ABL⁺ cells. Left panel displays colony numbers of one representative experiment out of two. Right panel shows photographs of a representative wild type and p27-Y88F colony formation assay. B Cells isolated from colonies were subjected to western blot. p27, v-ABL and SKP2 protein levels were analyzed. HSP90 was used as loading control. Right panel presents densitometric analysis of p27 protein levels normalized to HSP90. Data represent mean ± SD. (**p < 0.01). C p27-Y88F or p27-Y88F-CK⁻ expression was induced with 1 μg/ml doxycycline in HeLa Flp-In cells for 24, 72 and 96 h and p27, SKP2 and HSP90 protein levels were analyzed by western blot. One representative experiment out of three is shown.