. 2022 Jun 22;36(7):1703–1719. doi: 10.1038/s41375-022-01613-1

Table 13.

Lineage assignment criteria for mixed-phenotype acute leukaemia.

	Criterion
B lineage
CD19 strong^a	1 or more also strongly expressed: CD10, CD22, or CD79a^c
or,
CD19 weak^b	2 or more also strongly expressed: CD10, CD22, or CD79a^c
T lineage
CD3 (cytoplasmic or surface)^d	Intensity in part exceeds 50% of mature T-cells level by flow cytometry or, Immunocytochemistry positive with non-zeta chain reagent
Myeloid lineage
Myeloperoxidase	Intensity in part exceeds 50% of mature neutrophil level
or,
Monocytic differentiation	2 or more expressed: Non-specific esterase, CD11c, CD14, CD64 or lysozyme

^aCD19 intensity in part exceeds 50% of normal B cell progenitor by flow cytometry.

^bCD19 intensity does not exceed 50% of normal B cell progenitor by flow cytometry.

^cProvided T lineage not under consideration, otherwise cannot use CD79a.

^dUsing anti-CD3 epsilon chain antibody.