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. 2021 Nov 4;43(7):1803–1815. doi: 10.1038/s41401-021-00783-5

Fig. 5. Exogenous expression of Myc protects BAP1-deficient M14 cells from OTX015 inhibition, while ectopic expression of RING1A/1B enhances the sensitivity of M14 cells to OTX015.

Fig. 5

M14 BAP1 KO clones (#1 and #2) were stably transduced with control (pQCXIH-vec), Myc (pQCXIH-HA-Myc) (a) or Bcl-2 (pQCXIH-HA-Bcl-2) (c) retroviruses. Myc and Bcl-2 protein levels were determined by immunoblot analysis. Ectopic expression of Myc (b) or Bcl-2 (d) rescued M14 BAP1 KO clones (#1 and #2) from the cytotoxic effects of OTX015 to different extents. Cells were treated with OTX015 at the indicated concentrations for 6 days. The data are presented as the mean ± SD (n = 3) from one representative experiment out of three. e Immunoblot analysis showing ectopic expression of RING1A and RING1B in M14 cells. Cells were stably transduced with control (pQCXIH-vec), RING1A (pQCXIH-MYC-RING1A), or RING1B (pQCXIH-HA-RING1B) retroviruses. f Ectopic expression of RING1A or RING1B in M14 cells augmented sensitivity to OTX015. Cells were treated with OTX015 at the indicated concentrations for 6 days. The data are presented as the mean ± SD (n = 3) from one representative experiment out of three.