Table 2.

Gradient elution steps.

Time (min)	Flow rate (mL/min)	Solvent A (%)	Solvent B (%)
0	0.5	95	5
1	0.5	90	10
8	0.5	55	45
12	0.5	45	55
13	0.5	5	95
15	0.5	95	5
16	0.5	95	5