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. 2021 Nov 22;43(7):1686–1698. doi: 10.1038/s41401-021-00806-1

Fig. 3. Clk1 deficiency increases plasma dopamine transporter (DAT) expression without alteration of dopamine D1 receptors.

Fig. 3

a, b WT or Clk1+/− mutant mice were sacrificed and striatum, hippocampal and prefrontal cortex tissues were dissected and processed for immunoblot assays of DAT. c, d At 72 h after LV-shNC or LV-shClk1 transduction, successfully infected PC12 cells were selected and cultured as described in Methods. Then, PC12 cells with stable expression of LV-shNC or LV-shClk1 was collected for immunoblot assays. e WT or Clk1+/− mutant mice were sacrificed and brain tissue collected for immunoblot assays of D1R in striatum, hippocampus and prefrontal cortex. Membrane proteins were isolated as described in Methods. For a, representative images for immunoblots are shown in the upper panels and quantitative data are shown in the lower panel. For each of b–e, representative images for immunoblots are shown in the left panels and quantitative data are shown in the right panels. Bar graphs are presented as means ± SEM, with 3 independent experiments conducted for each immunoblot assay. Statistical analyses were performed using two-way ANOVA followed by Bonferroni-corrected tests (*P < 0.05, ***P < 0.001).