Skip to main content
. 2021 Nov 22;43(7):1686–1698. doi: 10.1038/s41401-021-00806-1

Table 1.

Sequences of the primers used for RT-PCR.

Species Gene Primer Sequence
Mouse DAT Forward 5’-CTTCTCCTCTGGCTTCGTTGT-3'
DAT Reverse 5’-CAGGGTAGATGATGAAGATCAACC-3'
Mouse hepcidin Forward 5’-TTGCGATACCAATGCAGAAGAG-3'
hepcidin Reverse 5’-AATTGTTACAGCATTTACAGCAGAAGA-3'
Mouse TfR1 Forward 5’-GTTTCTGCCAGCCCCTTATTAT-3'
TfR1 Reverse 5’-GCAAGGAAAGGATATGCAGCA-3'
Mouse DMT1 Forward 5’-TCTATGTGGTGGCTGCAGTG-3'
DMT1 Reverse 5’-GCTGGTATCTTCGCTCAGCA-3'
Mouse FTL Forward 5’-CCATCTGACCAACCTCCGC-3'
FTL Reverse 5’-CGCTCAAAGAGATACTCGCC-3'
Mouse GAPDH Forward 5’-TGTGTCCGTCGTGGATCTGA-3'
GAPDH Reverse 5’-TTGCTGTTGAAGTCGCAGGAG-3’
Rat hepcidin Forward 5’-GAAGGCAAGATGGCACTAAGCA-3'
hepcidin Reverse 5’-TCTCGTCTGTTGCCGGAGATAG-3'
Rat TfR1 Forward 5’-CGAAGTCCAGTGTGGGAACA-3'
TfR1 Reverse 5’-TGGCACCAACAGCTCCATAG-3'
Rat DMT1 Forward 5’-GGCAGTGTTTGATTGCGTTG-3'
DMT1 Reverse 5’-TAGTATTGCCACCGCTGGTATC-3'
Rat FPN1 Forward 5’-ATGTATCATGGATGGGTCAAAAC-3'
FPN1 Reverse 5’-TACGACAACAATCCAGTCC-3'
Rat β-actin Forward 5’-CCCTAAGGCCAACCGTGAAAAG-3'
β-actin Reverse 5’-TACGTACATGGCTGGGGTGT-3'
Rat FTL Forward 5’-GATTCGTCAGAATTATTCCA-3'
FTL Reverse 5’-AGAGAGGTAGGTGTAAGA-3'
Rat Clk1 Forward 5’-CGGTTTTGATGCCCTTGTGG-3'
Clk1 Reverse 5’-AGGCCATTGCTCCTTCCTTC-3'