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. 2021 Nov 16;43(7):1816–1828. doi: 10.1038/s41401-021-00787-1

Fig. 3. Omeprazole targets the Snail protein and reduces its stability.

Fig. 3

a–c The expression of Snail in HCT116 cells transfected with control-shRNA, Snail-shRNA1 or Snail-shRNA2 was determined by immunoblotting, and the inhibitory activity of omeprazole on migration was quantified (n = 3). d The binding affinities of omeprazole (left) and pantoprazole (right) for Snail were measured by biolayer interferometry. e The expression of Snail in various cancer cells after omeprazole treatment was evaluated by immunoblotting. f Immunofluorescence staining of Snail in MMTV-PyMT (left) and HCT116 (right) cells after omeprazole treatment. g, h The expression of Snail in the presence of cycloheximide (CHX, 100 μg/ml) in HCT116 cells treated with omeprazole was evaluated by immunoblotting, and the level of Snail expression was quantified. The level of FLAG-tagged Snail in HEK293T cells treated with omeprazole for 48 h (i) and in cells treated with 0.2 mM omeprazole for different times (j) was evaluated by immunoblotting. All representative blots shown are from three independent experiments. Data are presented as the mean ± SD. One-way analysis of variance was used for statistical analysis. *P < 0.05; **P < 0.01; N.S. not significant vs. vehicle.