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. 2021 Nov 16;43(7):1816–1828. doi: 10.1038/s41401-021-00787-1

Fig. 4. Omeprazole promotes Snail degradation by disrupting CBP/p300-mediated Snail acetylation.

Fig. 4

a The ubiquitination level of FLAG-tagged Snail in HEK293T cells after omeprazole treatment was investigated by immunoprecipitation. b Ubiquitination assay of endogenous Snail protein in HCT116 cells treated with omeprazole. c The acetylation level of FLAG-tagged Snail in HEK293T cells treated with omeprazole. d Acetylation assay of endogenous Snail protein in HCT116 cells treated with omeprazole. e The binding interaction of exogenous Snail with CBP/p300 was evaluated in HEK293T cells treated with omeprazole. f The binding interaction of endogenous Snail with CBP/p300 was evaluated in HCT116 cells treated with omeprazole. g, h The effect of omeprazole on the association of CBP-HAT with Snail was assessed by a Streptavidin pull-down assay and quantified. All representative blots shown are from three independent experiments.