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. 2021 Nov 16;43(7):1816–1828. doi: 10.1038/s41401-021-00787-1

Fig. 5. Omeprazole induces cell proliferation inhibition by limiting Snail-dependent cell cycle progression.

Fig. 5

a CCK-8 cell proliferation assay of different cancer cell lines treated with omeprazole (n = 3). b CCK-8 analysis of control and Snail-silenced HCT116 cells after omeprazole treatment (n = 3). c Effect of omeprazole on the cell cycle distribution in HCT116 cells. X-axis—DNA content as measured by PI incorporation. Y-axis—cell counts for each phase of the cell cycle. d Quantitative analysis of the cell cycle distribution as described in c (n = 3). e Immunoblot analysis of Snail, Cyclin D1, CDK4, CDK6, and phospho-Rb in HCT116 cells treated with omeprazole. f Representative histogram of the cell cycle distribution in control cells (top) and two independent Snail-silenced HCT116 cell lines (middle and bottom). Cells were treated with nocodazole for 0, 4, 6, and 8 h before harvesting. g Quantitative analysis of the cell cycle distribution as described in f. h Immunoblot analysis of Snail, Cyclin D1, CDK4, CDK6, and phospho-Rb in control and two independent Snail-silenced HCT116 cell lines. i Immunoblot analysis of the indicated proteins in control and Snail-silenced HCT116 cells treated with omeprazole. All representative blots shown are from three independent experiments. Data are presented as the mean ± SD.