Fig. 6. Oral administration of omeprazole inhibits Snail-driven colon cancer xenograft growth.

Reductions in HCT116 xenograft tumor size (a) and weight (b) upon omeprazole treatment (n = 6). Mice were treated orally with omeprazole for 14 days starting when the tumor volume reached ~100 mm³. c The levels of Snail, Cyclin D1, CDK4, CDK6, and phospho-Rb in tumor lysates were analyzed by immunoblotting (n = 3 pools from six mice, each). d, e Immunohistochemical staining of Ki67, phospho-histone H3, Cyclin D1, and phospho-Rb in xenograft tumors; the staining intensity was quantified (n = 6). The size (f) and weight (g) of HCT116 xenograft tumors derived from control or Snail-silenced cells were monitored in mice treated orally with omeprazole for 14 days (n = 6). h Immunoblot analysis of Snail, Cyclin D1, CDK4, CDK6, and phospho-Rb in tumor lysates as described in f and g (n = 2 pools from six mice, each). Data are presented as the mean ± SD. One-way analysis of variance and two-tailed Student’s t test were used for statistical analysis. **P < 0.01 vs. vehicle.