Fig. 7. Omeprazole inhibits mammary tumor growth in MMTV-PyMT transgenic mice.

a Primary tumor growth inhibition upon omeprazole treatment in MMTV-PyMT mice (n = 6). Mice were treated orally with omeprazole for 27 days, starting when the total tumor volume reached ~400 mm³. b, c Immunohistochemical staining of Ki67, phospho-histone H3, and Cyclin D1 in primary tumors; the staining intensity was quantified (n = 6). d Representative photograph of H&E staining for the indicated primary tumors (n = 6). Magnified images of the boxed areas in the sections are shown in the bottom panels. e, f Immunofluorescence staining of CD31, VEGFA, and α-SMA in primary tumors; the staining intensity was quantified (n = 6). g Immunofluorescence staining of K14 in primary tumors (n = 6). Data are presented as the mean ± SD. Two-tailed Student’s t test was used for statistical analysis. **P < 0.01 vs. vehicle.