Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jul 4;13:3835. doi: 10.1038/s41467-022-31476-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 5 — a, b Microarray data of de novo serine synthesis genes and UTX in the kidneys of patients with chronic kidney disease (CKD; a) or diabetic kidney disease (DKD; b). c Representative images of PHGDH staining in the kidneys of patients with DKD (top) and obesity (bottom); n = 4-9; scale bar, 50 μm. Brown color indicates positive staining. d–g eGFR (d), serum serine level (e), correlation analysis between the serum serine level and serum CREA (creatinine, f) or serum BUN (blood urea nitrogen, g) level or eGFR (h) from healthy individuals (n = 39), overweight (n = 23), obesity (n = 24) and obesity-related renal disease (n = 12). d, e data shown as mean ± SD. ^*P_{eGFR (CT vs obesity)} = 0.0227, ^***P_{eGFR (CT vs obesity related renal dysfunciton)} < 0.0001, ^***P_{eGFR (overweight vs obesity related renal dysfunciton)} < 0.0001, ^***P_{eGFR (obesity vs obesity related renal dysfunciton)} < 0.0001; ^***P_{serine (CT vs obesity related renal dysfunciton)} = 0.0004, ^**P_{serine (overweight vs obesity related renal dysfunciton)} = 0.0022, ^*P_{serine (obesity vs obesity related renal dysfunciton)} = 0.0309 (one-way ANOVA). f–h correlation analysis was performed by Pearson’s method, the Pearson correlation coefficients and p values (two-tailed test) were shown. i Representative images of renal PHGDH staining of WT and Utx^Ksp KO mice under NC or HFD conditions. Scale bar, 50 μm. j Western blots of PHGDH and HSP70 levels in the renal medulla and cortex of indicated groups, n = 3 independent animals. k–n Serine and glycine levels in renal medulla (k), renal cortex (l), serum (m), and urine (n) of indicated groups, data shown as mean ± SD. k–l n = 6, 6, 7, 5 independent animals for WT + NC or Utx^Ksp KO + NC or WT + HFD or Utx^Ksp KO + HFD group, respectively; (k) ^***P_{serine (WT+NC vs WT+HFD)} = 0.0005, ^*P_{serine (WT+HFD vs Utx}^Ksp _KO+HFD) = 0.0126; m n = 5, 4, 5, 5 independent animals for WT + NC or Utx^Ksp KO + NC or WT + HFD or Utx^Ksp KO + HFD group, respectively, ^**P_{serine (WT+HFD vs Utx}^Ksp _KO+HFD) = 0.005; ^**P_{glycine (WT+NC vs WT+HFD)} = 0.0068, ^**P_{glycine (WT+HFD vs Utx}^Ksp _KO+HFD) = 0.0018; (n) n = 6, 5, 7, 5 independent animals for WT + NC or Utx^Ksp KO + NC or WT + HFD or Utx^Ksp KO + HFD group, respectively, ^***P_{serine (WT+NC vs WT+HFD)} = 0.0009; ^***P_{glycine (WT+NC vs WT+HFD)} < 0.0001, ^*P_{glycine (WT+HFD vs Utx}^Ksp _KO+HFD) = 0.033 (one-way ANOVA). Source data are provided in the Source Data file.