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. 2022 Jul 4;13:3835. doi: 10.1038/s41467-022-31476-0

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — a, b TG level (a) and transcriptional levels of TG synthesis/lipid storage related genes (b) in UTX knockout or UTX/PHGDH double knockout (DKO) HK-2 cells, a n = 6, 6, 4, 4 biological samples for Ctrl + CT or Ctrl+PA or UTX KO + PA or DKO + PA group, respectively (mean ± SD); b n = 3 biological samples per group (mean ± SD). ^***P_{TG (Ctrl+CT vs Ctrl+PA)} < 0.0001, ^***P_{TG (Ctrl+PA vs UTX KO+PA)} < 0.0001, ^***P_{TG (UTX KO+PA vs DKO+PA)} < 0.0001; ^***P_{MOGAT1 (Ctrl+CT vs Ctrl+PA)} < 0.0001, ^***P_{MOGAT1 (Ctrl+PA vs UTX KO+PA)} < 0.0001, ^***P_{MOGAT1 (UTX KO+PA vs DKO+PA)} = 0.0006; ^***P_{GPAM (Ctrl+CT vs Ctrl+PA)} < 0.0001, ^***P_{GPAM (Ctrl+PA vs UTX KO+PA)} = 0.0002, ^***P_{GPAM (UTX KO+PA vs DKO+PA)} < 0.0001; ^***P_{CIDEA (Ctrl+CT vs Ctrl+PA)} < 0.0001, ^***P_{CIDEA (Ctrl+PA vs UTX KO+PA)} < 0.0001, ^***P_{CIDEA (UTX KO+PA vs DKO+PA)} < 0.0001; ^*P_{CIDEC (Ctrl+CT vs Ctrl+PA)} = 0.0163, ^*P_{CIDEC (Ctrl+PA vs UTX KO+PA)} = 0.014, ^**P_{CIDEC (UTX KO+PA vs DKO+PA)} = 0.0014 (one-way ANOVA). c, d Cellular (c) and medium (d) serine (left) or glycine (right) level in UTX knockout or UTX/PHGDH double knockout HK-2 cells, n = 6 biological samples per group (mean ± SD). c ^*P_{serine (Ctrl+CT vs Ctrl+PA)} = 0.0109, ^**P_{serine (UTX KO+PA vs DKO+PA)} = 0.0039; ^**P_{glycine (Ctrl+PA vs UTX KO+PA)} = 0.0014; (d) ^*P_{serine (Ctrl+PA vs UTX KO+PA)} = 0.0299, ^*P_{serine (UTX KO+PA vs DKO+PA)} = 0.0415 (one-way ANOVA). e, f TG level (e) and transcriptional levels of TG synthesis/lipid storage related genes (f) in NCT-503 treated UTX knockout HK-2 cells, (e) n = 6, 6, 3, 3 biological samples for Ctrl+CT or Ctrl+PA or UTX KO + PA or UTX KO + NCT (L) + PA or UTX KO + NCT (H) + PA group, respectively (mean ± SD). ^***P_{TG (Ctrl+CT vs Ctrl+PA)} < 0.0001, ^***P_{TG (Ctrl+PA vs UTX KO+PA)} < 0.0001, ^**P_{TG (UTX KO+PA vs UTX KO+NCT (L)+PA)} = 0.0023, ^**P_{TG (UTX KO+PA vs UTX KO+NCT (H)+PA)} = 0.0023; (f) n = 3 biological samples per group (mean ± SD) ^***P_{MOGAT1 (Ctrl+CT vs Ctrl+PA)} = 0.0006, ^*P_{MOGAT1 (Ctrl+PA vs UTX KO+PA)} = 0.0135, ^*P_{MOGAT1 (UTX KO+PA vs UTX KO+NCT (L)+PA)} = 0.0348, ^***P_{MOGAT1 (UTX KO+PA vs UTX KO+NCT (H)+PA)} < 0.0001; ^***P_{GPAM (Ctrl+CT vs Ctrl+PA)} < 0.0001, ^***P_{GPAM (Ctrl+PA vs UTX KO+PA)} = 0.0001, ^***P_{GPAM (UTX KO+PA vs UTX KO+NCT (L)+PA)} < 0.0001, ^***P_{GPAM (UTX KO+PA vs UTX KO+NCT (H)+PA)} < 0.0001; ^**P _{CIDEA (Ctrl+CT vs Ctrl+PA)} = 0.0049, ^**P_{CIDEA (Ctrl+PA vs UTX KO+PA)} = 0.0073, ^*P_{CIDEA (UTX KO+PA vs UTX KO+NCT (L)+PA)} = 0.0495, ^*P_{CIDEA (UTX KO+PA vs UTX KO+NCT (H)+PA)} = 0.0470; ^***P_{CIDEC (Ctrl+CT vs Ctrl+PA)} = 0.0001, ^***P_{CIDEC (Ctrl+PA vs UTX KO+PA)} < 0.0001, ^**P_{CIDEC (UTX KO+PA vs UTX KO+NCT (L)+PA)} = 0.0046, ^*P_{CIDEC (UTX KO+PA vs UTX KO+NCT (H)+PA)} = 0.0243 (one-way ANOVA). g, h Cellular (g) and medium (h) serine (left) or glycine (right) level in NCT-503 treated UTX knockout HK-2 cells, n = 6 biological samples per group (mean ± SD). g ^***P_{serine (Ctrl+CT vs Ctrl+PA)} < 0.0001, ^**P_{serine (Ctrl+PA vs UTX KO+PA)} = 0.001, ^**P_{serine (UTX KO+PA vs UTX KO+NCT (L)+PA)} = 0.005, ^**P_{serine (UTX KO+PA vs UTX KO+NCT (H)+PA)} = 0.002; ^***P_{glycine (Ctrl+CT vs Ctrl+PA)} < 0.0001, ^***P_{glycine (Ctrl+PA vs UTX KO+PA)} < 0.0001, ^***P_{glycine (UTX KO+PA vs UTX KO+NCT (L)+PA)} < 0.0001, ^***P_{glycine (UTX KO+PA vs UTX KO+NCT (H)+PA)} < 0.0001; (h) ^*P_{serine (Ctrl+CT vs Ctrl+PA)} = 0.0153, ^*P_{serine (Ctrl+PA vs UTX KO+PA)} = 0.021, ^**P_{serine (UTX KO+PA vs UTX KO+NCT (L)+PA)} = 0.009, ^***P_{serine (UTX KO+PA vs UTX KO+NCT (H)+PA)} = 0.0005; ^*P_{glycine (Ctrl+CT vs Ctrl+PA)} = 0.0273, ^**P_{glycine (Ctrl+PA vs UTX KO+PA)} = 0.0022, ^*P_glycine _{(UTX KO+PA vs UTX KO+NCT (L)+PA)} = 0.0188 (one-way ANOVA). a–h At least three independent experiments were performed and similar results were obtained^. Source data are provided in the Source Data file.