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. 2021 Nov 9;43(7):1793–1802. doi: 10.1038/s41401-021-00782-6

Fig. 7. Inhibition of Nrf2 partially blocks the chondroprotective effects of CDDO-Im.

Fig. 7

a NO levels in chondrocyte culture medium pretreated with CDDO-Im (20 nM) with or without TNF-α (50 ng/mL) in the presence or absence of ML385 (20 μM) for 24 h. b Representative images and quantification analyses of TUNEL staining in chondrocytes pretreated with CDDO-Im (20 nM) with or without TNF-α (50 ng/mL) in the presence or absence of ML385 (20 μM) for 24 h. c Representative images of immunofluorescence staining of MMP13 in chondrocytes pretreated with CDDO-Im (20 nM) with or without TNF-α (50 ng/mL) in the presence or absence of ML385 (20 μM) for 24 h. d Representative images of ROS in chondrocytes pretreated with CDDO-Im (20 nM) with or without TNF-α (50 ng/mL) in the presence or absence of ML385 (20 μM) for 2 h. e Quantitative analysis of the data in Fig. 7c and d. The values are presented as the means ± SDs. *P < 0.05 versus control, #P < 0.05 versus TNF-α-treated chondrocytes, †P < 0.05 versus TNF-α and CDDO-Im treated chondrocytes.