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. 2022 Jul 4;5:657. doi: 10.1038/s42003-022-03604-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Blots of lysates of S. cerevisiae strains expressing the indicated Asc1 fusion proteins and incubated or not (−) with 10 μM biotin for the indicated times. The fusion proteins were expressed from plasmids in a Δasc1 strain, and the ASC1 wild-type strain carrying the empty vector served as a control. Biotinylation was analyzed using HRP-labeled streptavidin and expression levels of the fusion proteins with antibodies against Asc1 as indicated. b Blots of lysates of E. coli cells transformed with expression plasmids for ultraID, microID, TurboID, or the control plasmid. The cells had been incubated (+) or not (−) with biotin for 16 h. The arrowhead indicated the expected size for bacterial GAPDH, and the asterisk (*) indicates a non-specific cross-reactivity that was used as a loading control.