Skip to main content
. 2022 Jul 4;8:22. doi: 10.1038/s41526-022-00209-4

Fig. 2. Simulated microgravity (sμg) enhances the self-renewal of hPSCs.

Fig. 2

Culture under sμg maintained self-renewal of hPSCs and enhanced the expression of the core set of pluripotent transcription factors (TF), transmembrane glycoproteins ITGA6 and ITGB1, and telomere genes. A Representative immunofluorescent micrographs of colonies from sμg and 1g conditions stained for the core set of pluripotent TFs. Scale bars, 200 µm. Graphs show the mean fluorescence intensity analysis from micrographs. B Representative immunoblots and densitometry analysis from protein lysates of cells cultured at sμg and 1g conditions. RT-qPCR analysis indicates relative mRNA levels of (C) pluripotent associated and (D) telomere elongation genes present in cells cultured at sμg and 1g conditions. *p < 0.05, **p < 0.005 (n = 3; unpaired t test). Error bars in graphs represent the SEM of the group.