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. 2022 Jul 4;8:22. doi: 10.1038/s41526-022-00209-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — hPSCs were cultured under sμg or 1g for 48 h with a medium that sustains their self-renewal, followed by a further 48 h of culture with a differentiation medium to induce (A) trophectoderm and (B) neuroectoderm differentiation. RT-qPCR analysis of representative genes related to pluripotency, trophectoderm, and neuroectoderm indicated that differentiation under sμg condition is reduced compared to 1g condition. *p < 0.05, **p < 0.005 (n = 3; unpaired t test). Error bars in graphs represent the SEM of the group.