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. 2022 Jul 4;8:22. doi: 10.1038/s41526-022-00209-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Representative immunofluorescent micrographs of hPSCs cultured in sμg and 1g conditions and stained for PSMD11 and SOX2. DAPI was used to stain the nuclei of all cells. Scale bars, 200 µm. Graphs showing the mean fluorescence intensity analysis from micrographs. B Representative Immunoblots and densitometry analysis of PSMD11 from protein lysates of cells cultured at sμg and 1g conditions. GAPDH was used as a loading control. Graphs on the right show the densitometry analysis of the immunoblots. C RT-qPCR analysis indicating the relative mRNA levels of PSMD11 present in cells cultured at sμg compared to 1g condition. *p < 0.05 (n = 3; unpaired t test). Error bars in graphs represent the SEM of the group.