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. 2022 Jul 4;12:11264. doi: 10.1038/s41598-022-14429-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of NACs after a triggering stimulus. (a) Schematic representation of the time points used for expression analysis of NACs in triggered plants (T), and in plants that were primed before triggering (P + T). In triggered (T) plants, the expression was calculated as a ratio of HS samples compared to unstressed controls. In primed and triggered (P + T) plants, the expression was calculated as primed and triggered (P + T), compared to triggered only (T). (b) K-means clustering of NAC expression profiles of primed and triggered (P + T) and triggered (T) plants after the triggering stimulus (k = 10 clusters). The expression of genes in each cluster is displayed as a heat map. The color indicates the expression as log2-fold change for the different time points, where yellow denotes an increased expression and purple a reduced expression. The list of genes in each cluster and their expression values are shown in Supplementary Table S2.